Perfusion double-channel micropipette probes for oxygen flux mapping with single-cell resolution

• Yang Gao,
• Bin Li,
• Riju Singhal,
• Adam Fontecchio,
• Ben Pelleg,
• Zulfiya Orynbayeva,
• Yury Gogotsi and
• Gary Friedman

Beilstein J. Nanotechnol. 2018, 9, 850–860, doi:10.3762/bjnano.9.79

• as individual cells at subcellular resolution. Keywords: double-barrel pipette; hydrodynamic confinement; perfusion; oxygen flux; single-cell metabolic analysis; Introduction Transport, production and consumption of gasses, ions, and organic molecules are fluxes that sustain life. Relatively few